Ph.D. Molecular and Cell Biology |
2008-2012 Department of Virology, Franche-Comté, Besançon FRANCE University of |
RESEARCH EXPERIENCE: During my research career, I have worked on following projects:
- Implication of Akt signaling in HIV-1 persistence and formation of viral reservoirs in CD4+ T cells during chronic viral infection
- HIV-1 pathogenesis in HIV-1/HCV coinfection .
- Oncomodulatory role of HCMV in liver cancer and role of HDAC inhibitors in rheumatoid arthritis:
SCIENTIFIC SKILLS
Molecular Biology: General: DNA/RNA isolation, PCR & Real time PCR, Recombinant DNA technology/Gateway cloning, Transgenesis in Drosophila melanogaster, Mapping of transgene. Protein: Protein expression and protein purification, protein-protein interaction (GST-pull down assay, immunoprecipitation), DNA- protein interaction (electrophoretic mobility shift assay), isolation of protein from cytoplasmic fraction, mitochondrial fraction and nuclear fraction, Immunoblotting/SDS-PAGE.
Virology: FMD viral isolation from clinical samples, genotyping of FMDV, HIV-1 drug testing, HIV-1/HCV viral propagation, quantification and infection using molecular clones; viral RNA isolation, quantification of HIV-1 reservoirs, Roche molecular Diagnostic (COBAS-AmpliPrep and Taqman 48 HIV Test)
Cell Biology: Animal cell culture: Primary cells- isolation of PBLs and Monocytes from buffy coats, culturing human monocytederived macrophages (MDM), and monocytic cell lines (U937), hepatocytes etc; protein expression and purification in mammalian cell lines, siRNA transfection, Flow cytometry: Apoptosis, Immunophenotyping, Fluorescent protein analysis, Cell sorting Immunological assays: ELISA, Cell proliferation/toxicity assays, Fluorescent microscopy, immunohistochemical staining.
Bioinformatics Skills Comparative genomics, Homology modeling, Phylogentic tree construction, Multiple sequence alignment, BLAST, protein/DNA analysis tools, Primer designing, Familiar with “Deep view”, “Bioedit” and “Jalview”. Computer skills: Competent with most of Microsoft office programs and knowledge of the principal software for molecular biology. |