Qualifications:

• B.Sc. ( Hon) 1995 ( Microbiology, Biochemistry, Physiology ), University of Karachi, Karachi, Pakistan
• M.Sc.    1996       (Microbiology) University of Karachi, Karachi, Pakistan.
• M.Phil 2008        (Biotechnology) Quaid-i-Azam University Islamabad, Pakistan.

Research interests:

• Environmental  Risk Assessment ; toxicity , genotoxicity  and nano toxicology  
• Toxicological based biosafety evaluation of GM crops, nutraceutical & herbal products
• Molecular diagnosis of veterinary pathogens i.e PPRV, FMDV, Brucellosis

Research activities:

Currently working as Principal Scientist at SEBD –NIBGE the Environmental Risk Assessment and Molecular Diagnostic group. This group is working under two themes; Environmental risk assessment and Molecular diagnosis of economically important veterinary diseases.  Being a part and lead the group involve in several different projects;  

• Analysis of pesticides formulations for toxicity testing using various tests i.e acute oral LD₅₀ in mice/rats (mg/kg), percutaneous LD₅₀ in albino rabbits (mg/kg), eye &dermal irritation in rabbits and acute fish toxicity LC₅₀.
• Mutagenicity assessment of various pesticides, nanoparticles and others compounds.
• Established an aquatic toxicity lab for acute fish toxicity (LC₅₀) using various fish species.
• In vitro genetic toxicological studies of bio pesticides using cultured cells mouse lymphoma (L5178Y Tk+/-) and Human cells (HepG2 liver cells).
• Cytotoxic, genotoxic and mutagenic potential of biological based pesticides in mouse cells (L 5178Y) and Human cells           (HepG2 liver cells) by using MT & Blue assay, comet, micronuclei, mouse lymphoma and pig-A assay.
• Toxicological based biosafety analysis of transgenic wheat with phytase gene & transgenic sugar having gene; AVP1, DRB1A & ATNHX1 and nutraceuticals (enzymes mixtures for poultry feed).
• Microbial screening method was optimized for the detection of oxytetracycline (tetracyclines group), ciprofloxacin &enrofloxacin (fluoro- quinolones) and neomycin sulfate (aminoglycoside) residues in spiked and chicken using B. subtilis and E. coli strains.
• Molecular diagnosis of Peste des petitis ruminant virus (PPRV) and Foot and mouth disease virus (FMDV).
• Optimized Loop Mediated Isothermal Amplification (LAMP) assay for PPRV detection in clinical and field samples (under CRP-IAEA project).
• Cell culture facility optimized in lab using cell line CHS-20 (Flp-In-CV-1, expressing Slam; Monokey CV cell with Slam receptor) from Vienna under the project CRP-IAEA for the isolation of PPRV and toxicity testing.

Projects:

Contributed in 3 projects as a member&Co P. I.

• Development of rapid and sensitive test for on-site diagnosis of Peste des Petits Ruminants disease (Funded by AIP-PARC). As Co P.I
• One health approach for molecular diagnosis, epidemiology and control of brucellosis in Pakistan (Funded by ALP-PARC).
• CRP-IAEA funded project on “Establishment of PCR based methods for early and sensitive diagnosis of peste des petite ruminants PPR”. 2007-2012, Funding by IAEA. 

Publications (last five years):

1. Bhatti F, Asad, S., Khan, Q. M., Mobeen, A., Iqbal, M.J., Asif, M., (2019) Risk assessment of genetically modified sugarcane expressing AVP1 gene. Food and Chemical Toxicology, 130: 267-275
2. Bhatti, F., Asad, S., Khan Q. M., Mobeen, A., Asif, M., (2018) Sub-chronic toxicological evaluation of genetically modified AVP1 sugarcane in albino rabbits Inter. J. Biosciences.  13 (2) 274-288.
3. Ashraf, W., Kamal, H., Mobeen, A, Waheed, U., Unger, H., and Khan, Q.M., (2016) “Loop-mediated Isothermal Amplification assay for rapid and sensitive detection of peste des petisruminants virus in field conditions” The Journal of Animal and Plant Sciences (JAPS).
4. Ashraf, W., Unger, H., Haris, S., Mobeen, A., Farooq, M., Asif, M. and Khan, Q.M., (2016). Genetic detection of peste des petitsruminants virus under field conditions: a step forward towards disease eradication. BMC veterinary research, 13(1, p34).
5. Sadiq, R., Khan, Q.M., Mobeen, A, Hashmat, A. J., (2015). In vitro toxicological assessment of iron oxide, aluminium oxide and copper nanoparticles in prokaryotic and eukaryotic cell types. Drug ChemToxicol. 2015 Apr; 38(2):152-61.
6. Ashraf, W., Khan, Q.M.,Mobeen, A, Kamal, H., Jalees, M., Farooq, M., Diallo, A., and Unger, H., (2015) Molecular detection and genotyping characterization of PPRV in various areas of Pakistan, 2007 to 2012. Bulletin empress, Animal health 360 (45).
7. Ismail, M., Khan, Q. M., Ali, R., andMobeen A., (2014). Genotoxicity of Chlorpyrifos in Freshwater FishLabeorohita using Alkaline Single-Cell Gel Electrophoresis (Comet) Assay. Drug ChemToxicol. October 2014, Vol. 37, No. 4 : Pages 466-471
8. Shehzadi, M., Afzal, M., Khan, M.U., Islam E., Mobeen, A., Anwar, S., Khan, .M., (2014).Enhanced degradation of textile effluent in constructed wetland system using Typhadomingensis and textile effluent-degrading endophytic bacteria. Water Research 58(2014)152-159
9. Ismail, M., Khan, Q.M., Ali, R., Ali, T. and Mobeen, A. (2014) Evaluation of the Genotoxicity of Chlorpyrifos in Common Indus Valley Toad, BufostomaticusUsing Alkaline Single-Cell Gel Electrophoresis (Comet) Assay. Agricultural Sciences, 5(4) 376-382.