Dr. Romana Tabassum (PS, Group Leader)
View Profile

Objectives:

• Production, purification and characterization of industrial enzymes ? -amylase and Protease.
• Molecular characterisation of industrial enzyme producing strains.
• Molecular biology of industrial enzymes alpha amylase and protease with reference to gene cloning and expression.
• Sequence analyses and comparison of predicted amino acid sequence of polypeptide for 3- D structure analysis.
• Enzyme kinetic and thermodynamic studies of enzymes
• Pilot Plant fermentation studies for production of  ? -amylase and protease for Industrial application.
• Directed Evolution of industrial enzymes for improvement of enzyme characteristics.

Bioenergy from Agroindustrial Wasteas

Objectives:

• Identification and development of micro flora for methane production.
• Bioconversion of agriculture and industrial wastes for methane production.
• Molecular biology of methane production.
   

Alpha-amylase (1, 4-a-D-glucan-4-glucanohydrolas; EC 3.2.1.1) degrade a-1, 4 glucosidic linkages in starch, glycogen and related polysaccharides to release malto-oligosaccharides and glucose in the alpha-anomeric form. The global market for starch processing enzymes is around US $156 million and the cost of the enzymes used in liquefaction process represents 24% of the total process cost. Proteases represent one of the three largest groups of industrial enzymes and account for about 60% of the total worldwide sale of enzymes.  Alpha amylase and protease has application in textiles, starch syrups, laundry, dishwashing, detergents, paper desizing, fermentation of ethanol, animal feed, leather and dairy industries.

Recent advances in recombinant DNA technology have permitted enzymes with improved working parameters to be applied for commercial production. This way, the starch industry will has enhanced plant efficiency, lower operating costs and production of high quality products.

Therefore, the local production of thermostable enzyme will have a direct impact in the process, performance, economics and feasibility.

Objectives:

• Production, purification and characterization of industrial enzymes -amylase and protease.
• Molecular characterisation of industrial enzyme producing strains.
• Molecular biology of industrial enzymes alpha amylase and protease
• Sequence analyses and comparison of predicted amino acid sequence of polypeptide for 3- D structure analysis.
• Enzyme kinetic and thermodynamic studies of enzymes
• Pilot Plant fermentation studies for production of  -amylase and protease.
• Mass transfer of oxygen for enzyme production. 
• Directed Evolution of industrial enzymes for improvement of enzyme characteristics

Research Grants

Publications

Thesis Work