The main objective of the group will be to structurally and functionally characterize physiologically important human membrane proteins and of course their homologues of bacterial origin affected during the advent of different diseases. The characterization of these proteins will provide an insight into the transport mechanism of relevant substrates across cell membrane and the basis of construction of three dimensional homology models. This will ultimately lead towards drug discovery with following objectives:

• Development of strategies for cloning and high level expression of membrane and soluble proteins (drug targets or metabolically important) from both prokaryotes and eukaryotes.
• Purification of proteins by affinity, ion exchange and size exclusion chromatography.
  Biochemical characterization of proteins using radio or fluorescent probe labelled substrates in vitro and in vivo.
• Crystallization (3 dimensional) of homogenously purified proteins and determination of their structure by X-ray diffraction methods.